What are the enzymes in DNA replication?

DNA replication requires other enzymes in addition to DNA polymerase, including DNA primase, DNA helicase, DNA ligase, and topoisomerase.

What are nucleic acids show DNA replication?

DNA Replication is required for all cell division, which allows organisms to grow. In DNA replication, the DNA is first divided into two daughter strands in the genome, which carries the exact genetic information as the original cell.

How does DNA replication terminate?

Termination of DNA replication occurs when two replication forks meet on the same stretch of DNA, during which the following events occur, although not necessarily in this order: forks converge until all intervening DNA is unwound; any remaining gaps are filled and ligated; catenanes are removed; and replication …

What is the last step in DNA replication called?

In the final stage of DNA replication, the enyzme ligase joins the sugar-phosphate backbones at each nick site. After ligase has connected all nicks, the new strand is one long continuous DNA strand, and the daughter DNA molecule is complete.

How does DNA replication termination in eukaryotes?

In eukaryotic cells, termination of replication usually occurs through the collision of the two replicative forks between two active replication origins. The location of the collision varies on the timing of origin firing.

How does DNA polymerase replicate the template strand?

DNA polymerase must replicate the template strand behind the origin of replication. This is achieved by adding short segments of nucleotides to the newly exposed sections from the fork towards the origin of replication. The section of the DNA strand behind the origin of replication is known as the ‘lagging strand’.

How is the replication of DNA semiconservative?

DNA replication is semiconservative, meaning that each strand in the DNA double helix acts as a template for the synthesis of a new, complementary strand. This process takes us from one starting molecule to two “daughter” molecules, with each newly formed double helix containing one new and one old strand.

How often are mistakes made in the replication of DNA?

The replication of DNA is an incredibly fast and accurate process. On average, around one mistake is made for every 10 billion nucleotides that are replicated. The process includes over a dozen different types of enzymes and other proteins to run correctly.

How are proteins associated with the replication fork?

A number of proteins are associated with the replication fork to help in the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new strands by adding nucleotides that complement each (template) strand. DNA replication occurs during the S-stage of interphase .