How are recombinant inbred lines developed?

How are recombinant inbred lines developed?

RECOMBINANT inbred lines (RILs) can serve as powerful tools for genetic mapping. An RIL is formed by crossing two inbred strains followed by repeated selfing or sibling mating to create a new inbred line whose genome is a mosaic of the parental genomes (Figure 1).

What is the purpose of recombinant inbred lines?

Recombinant inbred lines (RILs) are a collection of strains that can be used to map quantitative trait loci. Parent strains are crossed to create recombinants that are then inbred to isogenicity, resulting in a permanent resource for trait mapping and analysis.

What is the difference between pure line and inbred line?

The term “pure line” is sometimes incorrectly applied to inbred lines, which are the progeny of animals or cross-pollinated plants obtained from a single pair of ancestors and maintained over a number of generations by constant crossing of related individuals and by selection. …

What is meant by inbred lines?

From Wikipedia, the free encyclopedia. Inbred strains (also called inbred lines, or rarely for animals linear animals) are individuals of a particular species which are nearly identical to each other in genotype due to long inbreeding.

Why is it important to develop inbred lines for hybrid cultivar development?

The breeder’s goal is to ultimately develop inbred lines with performance that results in an improvement over both of the original parents. The breeder accomplishes this thru repeated cycles of selection of plants with the best combination of characteristics and selfing.

How do you maintain inbred lines?

The nucleus seed of inbred lines can be maintained by self pollination, sib-pollination, or a combination of the two procedures (hand pollination). Some breeders prefer ‘sibbing” because it maintains vigour. “Selfing” is used to stabilize inbred lines if a change in breeding behavior is noticed.

How many single crosses are possible if there are 8 inbred lines?

Each line was crossed with eight other lines in a system described as an incomplete diallel series in such a way that there was a total of 116 single crosses.

Which country is the most inbred?

Data on inbreeding in several contemporary human populations are compared, showing the highest local rates of inbreeding to be in Brazil, Japan, India, and Israel.

What is inbred seed?

When farmers plant an entire field to a single variety, they produce inbred seed. It is referred to as the female and accepts pollen from the male parent to produce the hybrid seed.

How is the Drosophila melanogaster synthetic population resource created?

The Drosophila Synthetic Population Resource (DSPR) consists of a new panel of over 1700 recombinant inbred lines (RILs) of Drosophila melanogaster, derived from two highly recombined synthetic populations, each created by intercrossing a different set of 8 inbred founder lines (with one founder line common to both populations).

What is the history of recombinant inbred strains?

The origins and history of recombinant inbred strains are described by Crow. While the potential utility of recombinant inbred strains in mapping analysis of complex polygenic traits was obvious from the outset, the small number of strains only made it feasible to map quantitative traits with very large effects (quasi-Mendelian loci).

How is the dspr used in the Drosophila community?

The DSPR has been developed as a community resource for high-resolution QTL mapping and is intended to be used widely by the Drosophila community. Here you can track the progress of the development of the DSPR and find information regarding: the DSPR in general, our analytical machinery, and how to obtain the RILs and/or genomic data.

How many families of inbred strains are there?

Families of recombinant inbred strains numbering from 25 to 5000 are often used to map the locations of DNA sequence differences ( quantitative trait loci) that contributed to differences in phenotype in model organisms.