What are the Diff-Quik stains?
What are the Diff-Quik stains?
The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying.
What is Diff-Quik fixative solution?
RAL Diff-Quik fixative solution It fixes air-dried blood smears. This methanol based solution will stabilize cellular components. Solution I and II are applied individually to the fixed smear to differentially stain specific cellular components.
How do you make Diff Diff-Quik?
- Allow smears to dry.
- Dip slide or tape-strip five times, for one second each, into Fixative.
- Dip slide or tape-strip five times, for one second each, into Stain 1.
- Dip slide or tape-strip five times, for one second each, into Stain 2.
- Rinse slide or tape-strip in distilled water or Weise’s buffer, pH 7.2.
What are Diff Quick stains used for?
Diff-Quik is a commercial Romanowsky stain variant used to rapidly stain and differentiate a variety of pathology specimens. It is most frequently used for blood films and cytopathological smears, including fine needle aspirates.
How do you use Diff Quick?
Diff-Quik solutions should be stored in air tight containers to avoid evaporation or spillage. Start by opening all three pots. Gently dip the slide into staining pot 1 (Fixative solution) for one second… … and remove. Repeat – dipping the slide a total of 5 times, each time lasting 1 second.
What is Leishman stain procedure?
Leishman Stain is a neutral stain for blood smears which was devised by the British surgeon W. B. Leishman (1865–1926). It consists of a mixture of eosin (an acidic stain), and Methylene blue (a basic stain) in Methyl alcohol and is usually diluted and buffered during the staining procedure.
Why buffer is used in Leishman stain?
Besides the dyes, a buffer is added to the stain which acts as the mordant and enhances the staining reaction, results in the better morphology of the blood cells under the microscope.
What is the function of Leishman stain?
Leishman stain, also known as Leishman’s stain, is used in microscopy for staining blood smears. It is generally used to differentiate between and identify white blood cells, malaria parasites, and trypanosomas.
What makes up the chemical composition of air?
Nitrogen — N 2 — 78.084% Oxygen — O 2 — 20.9476% Argon — Ar — 0.934% Carbon Dioxide — CO 2 — 0.0314% Neon — Ne — 0.001818% Methane — CH 4 — 0.0002% Helium — He — 0.000524% Krypton — Kr — 0.000114% Hydrogen — H 2 — 0.00005% Xenon — Xe — 0.0000087% Ozone — O 3 — 0.000007%
What makes up most of the air in the atmosphere?
Updated September 21, 2018. Nearly all of the Earth’s atmosphere is made up of only five gases: nitrogen, oxygen, water vapor, argon, and carbon dioxide. Several other compounds are also present. Although this CRC table does not list water vapor, air can contain as much as 5% water vapor, more commonly ranging from 1-3%.
What kind of gas is in the air?
Although this CRC table does not list water vapor, air can contain as much as 5% water vapor, more commonly ranging from 1-3%. The 1-5% range places water vapor as the third most common gas (which alters the other percentages accordingly).
What are the main components of dry air?
Components in Dry Air Air is a mixture of several gases, where the two most dominant components in dry air are 21 vol% oxygen and 78 vol% nitrogen. Oxygen has a molar mass of 15.9994 g/mol and nitrogen has a molar mass of 14.0067 g/mol.