What is SYBR stain used for?
What is SYBR stain used for?
Invitrogen SYBR Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can be used with either blue-light or UV excitation.
Why is SYBR Safe better than ethidium bromide?
SYBR® Safe Its major advantage is that it is as sensitive as ethidium bromide but does not require UV light for visualization. Toxicity: Documented as less mutagenic that ethidium bromide, but its acute toxicity is higher.
How safe is SYBR Green?
In numerous tests carried out by independent, licensed testing laboratories, SYBR Safe DNA Gel Stain showed little or no genotoxicity and no acute toxicity.
How do you stain a SYBR Safe?
SYBR® Safe stain can be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR® Safe stain following electrophoresis. SYBR® Safe stain can be stored at room temperature in its original packaging to avoid excessive light exposure.
Is SYBR Safe toxic?
SYBR Safe DNA Gel Stain is nontoxic, noncarcinogenic, and classified as nonhazardous waste. SYBR Safe DNA Gel Stain in 0.5X TBE did not show any toxicity or mortality in an acute oral toxicity study: the rat LD50 was >5,000 mg/kg (U.S. EPA OPPTS 870.1100 method).
What does SYBR stand for?
SYBR
| Acronym | Definition |
|---|---|
| SYBR | Synergy Brands, Inc. (stock symbol) |
Is SYBR Safe Safe?
Safety. SYBR Safe is marketed as a safer alternative to ethidium bromide. However, as the molecule itself is quite a bit larger than ethidium bromide, it does not bind to the column of a gel extraction as easily, making it less efficient when trying to clone a DNA fragment into a plasmid.
What’s the difference between EtBr and SYBR Green?
For example, SYBR® Safe has very limited sensitivity while SYBR® Green and SYBR® Gold are much less stable than EtBr. In addition, many so-called “safe” DNA dyes like Midori Green, GreenSafe, SafeView™, and RedSafe™ not only have low sensitivity, but also readily penetrate living cells to bind DNA, and some are cytotoxic.
Is the SYBR Safe stain compatible with blue light?
SYBR Safe stain is compatible with blue light imaging systems as well as UV. Using blue light to visualize the DNA allows you to purify a band with virtually no UV-induced nicking or crosslinking. This can dramatically increase cloning efficiency.
Can a SYBR stain be used with ethidium bromide?
SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can be used with either blue-light or UV excitation. SYBR Safe stain is supplied as either a concentrate or a ready-to-use solution that can be used like an ethidium bromide solution.
How is SYBR Safe gel used in electrophoresis?
Easy to use. SYBR® Safe stain is supplied as a 10,000X concentrate in DMSO which can be used just like a solution of ethidium bromide. SYBR® Safe stain can be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR® Safe stain following electrophoresis.