What is cell count in body fluid?

Body fluid cell counting provides valuable information for the diagnosis and treatment of a variety of conditions. Chamber cell count and cellularity analysis by optical microscopy are considered the gold-standard method for cell counting.

Is the hemocytometer obsolete for body fluid cell counting?

The accuracy and precision for CSF cell counts reported by Fleming et al1 suggest that the “glass ceiling” for automated BF cellular analysis has been broken. Hemocytometers, long since abandoned for manual platelet counting, may soon disappear from clinical laboratories altogether.

How do you count CSF?

Cerebrospinal fluids (CSF) are manually counted in a hemocytometer chamber to get a WBC/Nucleated cell count and RBC count. A slide is made after centrifugation and stained. The stained slide is examined and 100 cells are counted and differentiated into cell types.

How do you count cells in a hemocytometer?

To count cells using a hemocytometer, add 15-20μl of cell suspension between the hemocytometer and cover glass using a P-20 Pipetman. The goal is to have roughly 100-200 cells/square. Count the number of cells in all four outer squares divide by four (the mean number of cells/square).

What are the 4 body fluids?

Body fluids are considered to be the interstitial fluids, saliva, tears, and gastric juices. They moisten the tissues, muscles, body organs and skin.

What is RBC in hematology?

Red blood cells (RBC) are made in the bone marrow and contain hemoglobin, a protein that carries oxygen to the tissues in the body. These cells are also known as erythrocytes. Red blood cell count is a blood test that measures your levels of erythrocytes.

What is total nucleated cell count?

The Total Nucleated Cell count or TNC is the test most often reported as a measure of the cell count after cord blood processing. The TNC count includes both living and dead cells. In the CFU test a small portion is watched under controlled conditions to see if stem cells divide and form colonies.

What does RBC in CSF indicate?

Red blood cell (RBC) count—normally no red blood cells are present in the CSF. The presence of red blood cells may indicate bleeding into the CSF or may indicate a “traumatic tap” – blood that leaked into the CSF sample during collection.

What does elevated RBC in CSF mean?

Finding red blood cells in the CSF may be a sign of bleeding. However, red blood cells in the CSF may also be due to the spinal tap needle hitting a blood vessel. Additional conditions which this test may help diagnose include: Arteriovenous malformation (cerebral) Cerebral aneurysm.

What does a hemocytometer measure?

The hemocytometer (or haemocytometer) is a counting-chamber device originally designed and usually used for counting blood cells. The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber.

How to calculate the cell count in a hemocytometer?

• Cells per ml = the average count per square x the dilution factor x 104 (count 10 squares) • Example: If the average count per square is 45 cells x 5 x104 = 2,250,000 or 2.25 x 106 cells/ml. • Total cell number = cells per ml x the original volume of fluid from which cell sample was removed.

What is the dilution factor of a hemocytometer?

Your dilution factor is 2.67. That is calculated by dividing 80 by 30. Please use this in the formula (dilution factor * total cells in 5 squares * 10000)/5. This will give you the number of cells per ml.

How are body fluid cell counts usually performed?

Body fluid cell counts are usually performed in a hemocytometer–a labor intensive and costly test. Procedures for the performance of hemocytometer cell counts as well as normal values for various fluids can be found in most hematology text books.

Are there any sources of error in the hemocytometer?

Hemocytometer counts are, however, subject to the following sources of error: Non-uniform suspensions: It is assumed that the volume of cell suspension placed in the chamber represents a truly random sample. This will not be a valid assumption unless the suspension is monodispersable and free of cell clumps.